Glucoamylase Enzyme Production by Aspergillus niger Fermentation and Cassava Starch as Substrate

Murni, Sri Wahyu and Buliandari, Anggita Abdi and Kusumawati, Meir Diana (2009) Glucoamylase Enzyme Production by Aspergillus niger Fermentation and Cassava Starch as Substrate. In: Prosiding Seminar Nasional Teknik Kimia “Kejuangan” Pengembangan Teknologi Kimia untuk Pengolahan Sumber Daya Alam Indonesia, 28 Januari 2009, UPN "Veteran" Yogyakarta.

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Abstract

Glucoamylase enzyme is widely used in many industries. In Indonesia, this enzyme is needed increasingly
for recent years and nowadays still imported with expensive cost. To overcome this problem, an alternative
cheaper process for enzyme production was developed. It was conducted by an experimental investigation
via batch fermentation by Aspergillus niger fungi with cassava starch as substrate. The fermentation broth in
a 500-ml flask was incubated and shaken at room temperature and the substrate was varied at different
concentrations. For several certain time interval, the broth was sampled and therefore its enzyme activity,
dry cell weight, and remaining substrate concentration was analyzed.
This research showed that the optimum condition for fermentation was obtained at substrate
concentration of 9% and initial pH of 6. At this condition, the fermentation after 7 days produced
glucoamylase enzyme with activity of 0.1355 Unit/ml. In addition, this experiment resulted in the kinetics
parameters including saturation constant (KS) of 0.287 g/100 ml, maximum growth rate (max) of 4.127day-1
,
and enzyme-to-substrate productivity (YP/S) of 0.3826 Unit/g starch.
Keywords: Glucoamylase enzyme, cassava starch, Aspergillus niger, enzyme activity

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	Glucoamylase enzyme, cassava starch, Aspergillus niger, enzyme activity
Subjects:	T Technology > TP Chemical technology
Depositing User:	Dra., M.T SRI WAHYU MURNI
Date Deposited:	18 Oct 2022 08:07
Last Modified:	18 Oct 2022 08:07
URI:	http://eprints.upnyk.ac.id/id/eprint/31377

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