RAMADANI, FITRIANI (2025) ANALISIS VARIASI GENETIK MELON (Cucumis melo L.) DENGAN METODE Random Amplified Polymorphic DNA. Skripsi thesis, UPN Veteran Yogyakarta.
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Abstract
ANALISIS VARIASI GENETIK MELON (Cucumis melo L.) DENGAN
METODE Random Amplified Polymorphic DNA
Oleh: Fitriani Ramadani
Dibimbing oleh: Bambang Supriyanta
ABSTRAK
Melon (Cucumis melo L.) merupakan tanaman hortikultura yang penting
bagi perekonomian Indonesia. Penelitian ini bertujuan untuk mengetahui variasi
genetik melon menggunakan metode Random Amplified Polymorphic DNA
(RAPD). Metode dilakukan melalui penelitian lapangan dan laboratorium.
Analisis laboratorium menggunakan teknik PCR-RAPD dengan 8 primer RAPD,
dan data dianalisis menggunakan perangkat lunak NTSYS-pc 2.1. Dari 10 primer
yang digunakan, diperoleh 69 lokus dengan 98,86% pita DNA bersifat polimorfik.
Primer BC-388 dan BC-551 terbukti paling efektif dalam mengungkap variasi
genetik 10 kultivar melon, timun suri, dan blewah. Berdasarkan dendogram,
genotipe melon terbagi dalam dua kelompok utama. Kelompok pertama terdiri
dari DGMC-2-5-16-1-5, DGMD-4-9-3-3-4, Pearl Lady, Dalmatian, dan Golden
Apollo dengan rata-rata kemiripan genetik 0,77. Kelompok kedua terdiri dari
Orange Melody, Silver Light, dan Yuki Lady dengan rata-rata kemiripan yang
sama. Galur DGMC-2-5-16-1-5 dan DGMD-4-9-3-3-4 yang telah melalui proses
seleksi hingga generasi lanjut menunjukkan kestabilan genetik dan berpotensi
sebagai kandidat tetua dalam program persilangan melon berikutnya.
Kata kunci : Melon, RAPD, Variasi Genetik, Dendogram, PCR
v
ANALYSIS OF GENETIC VARIATION OF MELON (Cucumis melo L.)
USING THE Random Amplified Polymorphic DNA METHOD
By: Fitriani Ramadani
Supervised By: Bambang Supriyanta
ABSTRACT
Melon (Cucumis melo L.) is an important horticultural crop for Indonesia's
economy. This study aimed to assess the genetic variation of melon using the
Random Amplified Polymorphic DNA (RAPD) method. The research was
conducted through field and laboratory studies. Laboratory analysis was
performed using PCR-RAPD with eight RAPD primers, and the data were
analyzed using NTSYS-pc 2.1 software. A total of 69 loci were amplified from 10
primers, with 98.86% of the DNA bands being polymorphic. Primers BC-388 and
BC-551 were the most effective in detecting genetic variation among 10 cultivars
of melon, timun suri, and blewah. Based on the dendrogram, the melon genotypes
were divided into two major groups. The first group consisted of DGMC-2-5-16
1-5, DGMD-4-9-3-3-4, Pearl Lady, Dalmatian, and Golden Apollo, with an
average genetic similarity of 0.77. The second group included Orange Melody,
Silver Light, and Yuki Lady with a similar average similarity. The breeding lines
DGMC-2-5-16-1-5 and DGMD-4-9-3-3-4, which have undergone selection up to
advanced generations, exhibited genetic stability and are potential parental
candidates for future melon crossing programs.
Keywords: Melon, RAPD, Genetic Variation, Dendrogram, PCR
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| Item Type: | Tugas Akhir (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Melon, RAPD, Genetic Variation, Dendrogram, PCR |
| Subjek: | S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Pertanian > (S2) Magister Agroteknologi |
| Depositing User: | Eko Yuli |
| Date Deposited: | 20 Nov 2025 02:11 |
| Last Modified: | 20 Nov 2025 02:11 |
| URI: | http://eprints.upnyk.ac.id/id/eprint/45600 |
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