MOLECULAR IDENTIFICATION OF SUSPECTED VIRAL DISEASE ATTACK IN PEPPER (PIPER NIGRUM L.) PLANTS IN BANJARNEGARA

RAHMA, DEWANTI AMANDA AULIA (2025) MOLECULAR IDENTIFICATION OF SUSPECTED VIRAL DISEASE ATTACK IN PEPPER (PIPER NIGRUM L.) PLANTS IN BANJARNEGARA. Skripsi thesis, UPN Veteran Yogyakarta.

	Text 2_Cover_134210187_Dewanti Amanda Aulia Rahma.pdf Download (217kB)
	Text 3_Abstrak_134210187_Dewanti Amanda Aulia Rahma.pdf Download (163kB)
	Text 4_Halaman Pengesahan_134210187_Dewanti Amanda Aulia Rahma.pdf Download (2MB)
	Text 5_Daftar Isi_134210187_Dewanti Amanda Aulia Rahma.pdf Download (152kB)
	Text 6_Daftar pustaka_134210187_Dewanti Amanda Aulia Rahma.pdf Download (126kB)
	Text 1_Skripsi Full_134210187_Dewanti Amanda Aulia Rahma.pdf Restricted to Repository staff only Download (1MB)

Abstract

The decline in black pepper (Piper nigrum L.) production in Banjarnegara is
suspected to be associated with viral disease, based on field symptoms resembling
mottel disease commonly linked to CMV(Cucumber Mosaic Virus) and PYMoV
(Pepper Yellow Mottle Virus). This study aimed to detect the presence of CMV and
PYMoV, characterize symptom in the field, and determine the intensity of plant
damage caused by these viruses. Symptomatic pepper plant samples were collected
from cultivation areas in Banjarnegara. Detection was performed using PCR for
PYMoV and reverse-transcription for CMV, utilizing specific primers for each
virus. The presence of the viruses was indicated by amplicons of expected DNA
fragment sizes observed on electrophoresis gels. Plant damage intensity was
assessed using a scoring method on samples selected based on a diagonal sampling
pattern.The results showed that no amplicons of the target sizes were obtained in
any of the samples. Electrophoresis using PYMoV primers revealed only smears,
while no bands appeared using CMV primers. These findings suggest the absence
of CMV and PYMoV in the tested samples, this indicating the possible involvement
of other virus or abiotic disorders. T-Test analysis indicated a significant difference
in damage intensity between plants suspected of viral infection and those affected
by other causes. Further studies employing broader molecular diagnostic are
recommended to identifying the actual pathogen that involved in the field.
Keyword: Banjarnegara, CMV, Detection, PCR, Piper nigrum L, PYMoV.

Item Type:	Tugas Akhir (Skripsi)
Additional Information:	DEWANTI AMANDA AULIA RAHMA (Penulis-134210187) ; Mofit Eko Poerwanto (Pembimbing)
Uncontrolled Keywords:	Banjarnegara, CMV, Detection, PCR, Piper nigrum L, PYMoV.
Subjek:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian > (S1) Agroteknologi
Depositing User:	A.Md Eko Suprapti
Date Deposited:	14 Oct 2025 06:38
Last Modified:	14 Oct 2025 06:38
URI:	http://eprints.upnyk.ac.id/id/eprint/44348

Actions (login required)

View Item