Murni, Sri Wahyu and Gunarto, Gunarto (2008) Produksi Poli--Hidroksibutirat (PHB) Menggunakan Bakteri Bulkhoderia cepacia. Eksergi: majalah Ilmiah Jurusan Teknik Kimia, 1 (IX). pp. 70-74.
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Abstract
Plastics have important roles nowadays. However, its non-biodegradable property potentially makes a great
problem because of the accumulation of its waste. Therefore, many researches about biodegradable plastics have
been developed. Poly--hydroxybutyrate (PHB) is an alternative material to produce biodegradable plastics. The
objectives of this research are to study the production of PHB by using Bulkhoderia cepacia bacteria and soluble
starch substrate and determine the kinetics parameters including maximum specific growth rate (maks),
saturation constant (Ks), and yield ratio of product-to-bacterial cell (YP/X). Fermentation was conducted at room
temperature by using Ramsay medium with soluble starch at certain concentrations. The starter of Bulkhoderia
cepacia incubated for 24 hours was inoculated as many as 10% by volume. The PHB product and dry cell weight
were analyzed at certain several time intervals. This experiment was repeated at variation of phosphate
concentrations.This research showed that the optimum cell growth and PHB production was obtained at soluble
starch concentration of 8 g/100 ml and growth period of 72 hours. At this condition, the results were the dry cell
weight of 7.5 mg/ml and PHB concentration of 0.0095 mg/ml. The values of kinetics parameters were maks of
0.01292 g/ml. h, Ks of 0.2854 g/ 100ml, YP/X of 0.00143. The accumulation of PHB was optimum at limited phosphate
concentration. It could be also concluded that Bulkhoderia cepacia was a non-effective bacteria to produce PHB.
Keywords: Poly--hydroxybutyrate (PHB), biodegradable plastics.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Poly--hydroxybutyrate (PHB), biodegradable plastics. |
| Subjects: | T Technology > TP Chemical technology |
| Depositing User: | Dra., M.T SRI WAHYU MURNI |
| Date Deposited: | 18 Oct 2022 08:13 |
| Last Modified: | 18 Oct 2022 08:13 |
| URI: | http://eprints.upnyk.ac.id/id/eprint/31398 |
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