Wijayani, Ari and Muafi, Muafi and Wahyurini, Endah and Endah, Rina (2015) REGENERATION CALLUS OF Chrysanthemum AFTER IRRADIATED RAY GAMMA FOR THE RESILIENCE OF PLAIN MEDIUM. Journal Techno, 01 (01). pp. 61-67. ISSN 977246114800
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Abstract
Development of chrysanthemum plants in the plains of the medium is still facing obstacles due to unfavorable climate. Until today the availability of seeds tolerant chrysanthemum grown in the plains of the medium is not maximized so that the necessary effort in order to increase the genetic diversity available genetic material as the material selection to obtain tolerant chrysanthemums grown in plain medium. Research in order to follow up the problems of availability of seeds tolerant chrysanthemums grown in the plains of the medium has been conducted by researchers at several stages, one of which is the induction of callus after gamma ray irradiation in vitro. Research has been conducted in tissue culture laboratory of the Faculty of Agriculture UPN "Veteran" Yogyakarta from February to June 2015. Media regeneration tested is ½ MS with the addition of IAA 0.1 mg / l; 0.2 mg / l; 0.3 mg / l; 0.4 mg / l and 0.5 mg / l. The results showed ½MS regeneration media with the addition of IAA 0.3 mg / l spur shoots emerge percentage (100%); while growing shoots (8.67 days), high shoots (3:10 cm) and number of buds (7:00). Treatment regeneration medium with kinetin 2 mg / l + IAA 0.5 mg / l gives the number of root growth (13) and root length (4:07 cm)
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > SB Plant culture T Technology > T Technology (General) |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Ir.MP ARI WIJAYANI |
| Date Deposited: | 06 Nov 2017 08:15 |
| Last Modified: | 06 Nov 2017 08:15 |
| URI: | http://eprints.upnyk.ac.id/id/eprint/13635 |
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