Isolation of Hydrogen Production Bacteria From Sludge of Anaerobic Biogas Reactor.

Mahreni, Mahreni and KHOLISOH, Siti Diyar and Pawignyo, Harso and Yanisworo, Wijaya Ratih (2015) Isolation of Hydrogen Production Bacteria From Sludge of Anaerobic Biogas Reactor. In: The second Onternational Conference on Green Agro Industry Resource Management for sustainable Future, Yogyakarta.

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Abstract

Abstract - Hydrogen-producing bacteria can be found in the activated sludge generated from anaerobik waste treatment processes. Microbes in it consists of acetogenesis, metanogenesis and hydrogen -producing bacteria such as Bacillus and Clostridium. The purpose of this study to isolate the hydrogen -producing microbes from the mix culture in the anaerobik activated sludge. Sludge from the anaerobik bioreaktor simmer for 2 hours to kill vegetative cells so that only spores are present. Then the spores cultured in the hydrogen media in anaerobik conditions, at a temperature of 65o C for 3 times 72 hours. Hydrogen gas generated during the breeding is analized using GC. Identification of the hydrogen producing bacteria is conducted using DNA tests. It’s performed by 16S rRNA gene sequence comparison with the data base available in the GenBank/EMBL/DDBJ / PDB through the Blast program, showed that all three isolates were identified as Bacillus citrulans. Keywords--- Anaerobik sludge, Bacillus, Biohydrogen, Clostridium, Lignocellulose.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Anaerobik sludge, Bacillus, Biohydrogen, Clostridium, Lignocellulose.
Subjects: T Technology > TP Chemical technology
Divisions: Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User: Dr Mahreni Mahreni
Date Deposited: 23 Apr 2019 02:41
Last Modified: 23 Apr 2019 02:41
URI: http://eprints.upnyk.ac.id/id/eprint/19428

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