GENETIC DIVERSITY OF PLAIN MEDIUM RESISTANT OF CHRYSANTHEMUM PLANLET PRODUCED OF GAMMA RAY IRRADIATION

Wijayani, Ari and Srilestari, Rina (2016) GENETIC DIVERSITY OF PLAIN MEDIUM RESISTANT OF CHRYSANTHEMUM PLANLET PRODUCED OF GAMMA RAY IRRADIATION. International Journal of Bioscience, Biochemistry and Bioinformatics, 6 (3). pp. 139-144. ISSN ISSN 2010-3638

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Abstract

Genetic diversity can be improved with the use of gamma ray irradiation to obtain plants that are resistant to the disease. Changes that occur can be analyzed at the beginning of plant growth using molecular analysis using RAPD technique (Random Amplified Polymorfism DNA. This study aims to determine the genetic diversity produced by gamma rays irradiated and given the poly ethilene glycol given in plantlets to increase chrysanthemum resistance to Medium plain. The study used four primers tested on four treatments that were not irradiated plantlets and induced the poly ethilene glycol, gamma rays irradiated 25 gray un induced poly ethilene glycol, irradiated and indued the poly ethilene glycol 15 and 30%. The results showed a decrease of control diversity of genetic 0.20 to 0.30 in 25 gray irradiation treatment with given the poly ethilene glycol 15%. The use of the RAPD technique to determine genetic diversity resulting from gamma ray irradiation on the level of plantlets. Gamma ray irradiation treatment causing mutations in all treatments tested. Plantlets of 25 gray irradiation induced with 15% poly ethilene glycol / l showed changes in DNA structure

Item Type: Article
Subjects: S Agriculture > SB Plant culture
Divisions: Faculty of Engineering, Science and Mathematics > School of Civil Engineering and the Environment
Depositing User: Ir.MP ARI WIJAYANI
Date Deposited: 30 Oct 2017 01:49
Last Modified: 30 Oct 2017 01:49
URI: http://eprints.upnyk.ac.id/id/eprint/13533

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