ISOLATION OF HYDROGEN PRODUCING BACTERIA FROM SLUDGE OF ANAEROBIC BIOGAS REACTOR

Mahreni, Mahreni and Ratih, Yanisworo Wijaya and Kholisoh, Siti Diyar and Pawignyo, Harso (2015) ISOLATION OF HYDROGEN PRODUCING BACTERIA FROM SLUDGE OF ANAEROBIC BIOGAS REACTOR. In: The Second International Conference on Green Agro-Industry (ICGAI), 4-6 Agustus 2015, Yogyakarta.

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Abstract

Hydrogen-producing bacteria can be found in the activated sludge generated from
anaerobic waste treatment processes. Microbes in it consists of acetogenesis,
metanogenesis and hydrogen-producing bacteria such as Bacillus and Clostridium. The
purpose of this study was to isolate, characterize and identify a hydrogen-producing
bacteria from sludge of anaerobic biogas reactor. Hydrogen production was analyzed by
a gas chromatograph (GC-Shimadzu). Organics acid and ethanol productions were
analyzed by HPLC, whereas reducing sugar was analysis by DNS method. Isolates
identification was done based on the 16S rDNA gene sequences. Five isolates bacteria
have been obtained from enrichment culture (culture C4) were developed using liquid
minerals medium (HM medium). Based on the examination on the similarities of the
isolates morphology obtained, three isolates have been selected. They were BYM1,
BYM2, and BYM3. According to the sequence of their 16S-rDNA, the BYM1, BYM2,
and BYM3 were identified as Bacillus circulans with 97% similarities. The three isolate
Hydrogen-producing bacteria can be found in the activated sludge generated from
anaerobic waste treatment processes. Microbes in it consists of acetogenesis,
metanogenesis and hydrogen-producing bacteria such as Bacillus and Clostridium. The
purpose of this study was to isolate, characterize and identify a hydrogen-producing
bacteria from sludge of anaerobic biogas reactor. Hydrogen production was analyzed by
a gas chromatograph (GC-Shimadzu). Organics acid and ethanol productions were
analyzed by HPLC, whereas reducing sugar was analysis by DNS method. Isolates
identification was done based on the 16S rDNA gene sequences. Five isolates bacteria
have been obtained from enrichment culture (culture C4) were developed using liquid
minerals medium (HM medium). Based on the examination on the similarities of the
isolates morphology obtained, three isolates have been selected. They were BYM1,
BYM2, and BYM3. According to the sequence of their 16S-rDNA, the BYM1, BYM2,
and BYM3 were identified as Bacillus circulans with 97% similarities. The three isolate
bacteria were then designated as Bacillus circulans BYM1, Bacillus circulans BYM2
and Bacillus circulans BYM3. The acid production by the isolate and their combination
was higher than the C4 culture. The mixed cultures Bacillus circulans BYW2- Bacillus
circulans BYW3 had higher ability to produce hydrogen than the C4 culture. The
fermentation by-products were ethanol, acetic acid and propionic acid, and acetic acid is
the major metabolite.
.
Keywords: biogas sludge, Bacillus, bio-hydrogen.

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	biogas sludge, Bacillus, bio-hydrogen.
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Dr.Ir,MSi YANISWORO RATIH
Date Deposited:	17 Apr 2023 06:23
Last Modified:	18 Apr 2023 06:42
URI:	http://eprints.upnyk.ac.id/id/eprint/34017

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