Produksi, Karakterisasi, dan Isolasi Lipase dari Aspergilus niger menggunakan Minyak Goreng Sawit sebagai Induser

Murni, Sri Wahyu (2015) Produksi, Karakterisasi, dan Isolasi Lipase dari Aspergilus niger menggunakan Minyak Goreng Sawit sebagai Induser. Eksergi, XII (2). pp. 1-4. ISSN 144o-394X

Text 8j 2015 eksergy vol 12 no 1 karakterisasi enzim lengkap.pdf Download (1MB)

Abstract

ABSTRAK: Tujuan penelitian ini adalah memproduksi, mengisolasi, dan mengkarakterisasi
lipase dengan induser minyak goreng sawit. Fermentasi submerged 1,4 L digunakan selama
proses produksi pada suhu kamar, dengan pH awal 7, kecepatan pengadukan 250 rpm,
kecepatan aerasi vvm, dan konsentrasi induser 3%-m/v minyak goreng sawit. Enzim
dikarakterisasi pada variasi temperature dan pH. Lipase dihasilkan secara optimum pada pH 7
dan suhu 30 °C dengan aktivitas 1,5 u/ml. Isolasi lipase menghasilkan 4 kali peningkatan
dengan menggunakan 90% amonium sulfat
Kata Kunci: Aspergilus niger; aktivitas enzim; lipase; minyak goreng sawit.
ABSTRACT: The objective of the research was to produce, isolate and characterise lipase
from Aspergillus niger, and therefore inducted it by using palm oil. The lipase enzyme was
produced through a batch fermentation process in a 1.4 liters-fermentor. Fermentation was
carried out at room temperature, initial pH of 7, the stirring speed of 250 rpm, aeration rate
of 1 vvm, and inducer concentration of 3%-m/v palm oil/ml. Enzymes was characterised at
several temperature and pH variations. The lipase showed the optimum performance at pH
of 7 and temperature of 30 °C with the activity of 1.5 U / ml. Isolation of lipase yielded a
4-times-increase in its activity by using 90% ammonium sulfate.
Keywords: Aspergillus niger; enzyme activity; lipase; palm oil

Item Type:	Article
Uncontrolled Keywords:	Aspergillus niger; enzyme activity; lipase; palm oil
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Dra., M.T SRI WAHYU MURNI
Date Deposited:	18 Oct 2022 08:15
Last Modified:	18 Oct 2022 08:15
URI:	http://eprints.upnyk.ac.id/id/eprint/31404

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