Produksi Enzim Selulase dari Aspergillus niger Secara Fermentasi dalam Medium Kultur Padat

Murni, Sri Wahyu and Gunarto, Gunarto and Sudewi, Titik and Jaka S., Anggara (2012) Produksi Enzim Selulase dari Aspergillus niger Secara Fermentasi dalam Medium Kultur Padat. In: Prosiding Seminar Nasional Teknik Kimia “Kejuangan” 2012 Pengembangan Teknologi Kimia untuk Pengolahan Sumber Daya Alam Indonesia, 6 Maret 2012, UPN "Veteran" Yogyakarta.

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Abstract

Bioethanol made from lignocellulosic biomass is currently being developed. The main obstacle
production of bioethanol from lignocellulosic materials is at the stage of the process of hydrolysis
of lignocellulosic material to form sugars. Enzymatic hydrolysis using cellulase enzyme is plagued
by an expensive price. In the previous studies have been carried out production of Trichoderma
reesei cellulase enzymes,in this research conducted using Aspergillus niger by solid substrate
fermentation method. Enzyme production studies conducted in two stages in, there were in the
aerated Erlenmeyer flask and rotary drum fermenter (RDF). The results showed that the
production of cellulase enzymes in the rice straw substrate has resulted in enzyme activity similar
to sawdust from the wood sengon. Water content effect on enzyme activity. The best results were
obtained on a substrate of rice straw at 60% moisture content and fermentation time of 6 days, the
CMC-ase activity of FP-ase was 0.00229 and 0.00095U /ml respectivelly. The best results in
sengon sawdust substrate is reached at 70% moisture content and fermentation time of 7 days,
obtained CMC-ase and FP-ase activity 0.00257 and 0.00133 U/ml respectivelly. Productivity of
the enzyme in the RDF is similar to the Erlenmeyer flask. At RDF, obtained CMC-ase and FP-ase
of 0.00243 and 0.00105 U / ml for rice straw substrate, and 0.00224 and 0.00125 U / ml for
sengon sawdust substrate.
Key words: aktivitas enzim, fermentasi, substrat padat, selulosa, selulase

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	aktivitas enzim, fermentasi, substrat padat, selulosa, selulase
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Dra., M.T SRI WAHYU MURNI
Date Deposited:	18 Oct 2022 08:19
Last Modified:	18 Oct 2022 08:19
URI:	http://eprints.upnyk.ac.id/id/eprint/31379

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