CELLULASE ENZYME PRODUCTION BY Trichoderma reesei THROUGH A SOLID SUBSTRATE FERMENTATION PROCESS

Murni, Sri Wahyu and Kholisoh, Siti Diyar (2011) CELLULASE ENZYME PRODUCTION BY Trichoderma reesei THROUGH A SOLID SUBSTRATE FERMENTATION PROCESS. In: Proceeding of the 1st ACIKITA International Conference of Science and Technology (AICST),, 25-27 July 2011, ACIKITA foundation Jakarta.

Text B18 Prosiding ACIKITA Int Conf 2011 Celllulose Enzyme Production dari Tricoderma lengkap.pdf Download (1MB)

Abstract

Shortage of energy is one of the serious problems facing the world today. Ethanol produced from
vegetable materials is one of the most prospective alternative energy to reduce the dependence on fuel
oil. Lignocellulosic biomass has enormous potential to be processed into ethanol. The main inhibitor in
the enzymatic saccharification of biomass for ethanol production is costly cellulase enzymes. Cellulase
production costs can be reduced by various approaches, for instance, using cheaper lignocellulosic
biomass substrate for fermentation and applying more efficient fermentation strategies, such as solid
state fermentation. (SSF). In this research, cellulase was produces by using a solid culture of
Trichoderma reesei FNCC 6012. Enzyme production was conducted in Erlenmeyer flask and rotary
drum fermenter (RDF). The results showed that the cellulase activity on rice straw substrate was
higher than the mahogany sawdust. Moisture content influenced on the activity of enzymes produced. The
best results obtained on rice straw substrate at 60% moisture content and 6 days fermentation, with the
activity of CMC-ase and FP-ase respectively 0.00584 and 0.00318 U / ml respectivelly. The best result on
mahogany sawdust was achieved at 70% moisture content and 7 days fermentation, with CMC-ase and
FP-ase activity of 0.00434 and 0.00125 U/ml respectivelly. Enzymes productivity of in RDF was
observed better than the flasks. It was obtained in RDF that CMC-ase and FP-ase activity was 0.00645 and
0.00334 U/ml, for rice straw substrate, and 0.00579 and 0.00302 U/ml for mahogany sawdust.
Keywords: Enzyme activity, fermentation, solid subtrate, cellulase, cellulose

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	Enzyme activity, fermentation, solid subtrate, cellulase, cellulose
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Dra., M.T SRI WAHYU MURNI
Date Deposited:	16 Aug 2022 05:37
Last Modified:	30 Dec 2022 03:28
URI:	http://eprints.upnyk.ac.id/id/eprint/30668

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