INDUKSI PERTUMBUHAN KALUS WORTEL (Daucus carota L.) DENGAN BERBAGAI KONSENTRASI 2,4-D PADA KOMPOSISI MEDIA MS SECARA IN VITRO

Tama, Hanada Yogia (2022) INDUKSI PERTUMBUHAN KALUS WORTEL (Daucus carota L.) DENGAN BERBAGAI KONSENTRASI 2,4-D PADA KOMPOSISI MEDIA MS SECARA IN VITRO. Other thesis, UPN "Veteran" Yogyakarta.

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Abstract

v
INDUKSI PERTUMBUHAN KALUS WORTEL (Daucus carota L.)
DENGAN BERBAGAI KONSENTRASI 2,4-D PADA KOMPOSISI MEDIA MS
SECARA IN VITRO
Oleh : Hanada Yogia Tama (134170145)
Dibimbing oleh : Endah Wahyurini, S.P., M.Si. dan Ir. Lagiman, M.Si.
ABSTRAK
Wortel (Daucus carota L.) merupakan tanaman penting bernilai ekonomi tinggi. Pada
perbanyakan tanaman wortel secara konvensional terdapat kendala, yaitu umbi mudah busuk
pada saat musim penghujan mudah terserang penyakit busuk pada umbi, busuk daun, adanya
serangan gulma yang menghambat pertumbuhan wortel, dan hasil umbi wortel yang tidak
seragam. Kendala perbanyakan tanaman wortel secara konvensional dapat diatasi dengan
menggunakan perbanyakan secara in vitro. Penelitian ini bertujuan untuk menentukan adanya
interaksi antara perlakuan 2,4-D dan komposisi media MS dalam menginduksi pertumbuhan
kalus wortel secara in vitro, menentukan konsentrasi 2,4-D yang tepat dalam menginduksi
pertumbuhan kalus wortel secara in vitro, dan menentukan komposisi media MS yang tepat
dalam menginduksi pertumbuhan kalus wortel secara in vitro. Penelitian dilaksanakan di
Laboratorium Bioteknologi UPN “Veteran” Yogyakarta. Metode penelitian yang digunakan
adalah Rancangan Acak Lengkap (RAL) dengan dua faktor. Faktor pertama adalah 2,4-D,
yaitu kontrol, 0,5 mg/l, 1 mg/l, dan 1,5 mg/l. faktor kedua adalah media MS, yaitu ¼ MS, ½
MS, dan MS. Data dianalisa menggunakan sidik ragam (ANOVA) dengan DMRT 5%. Hasil
penelitian menunjukkan bahwa perlakuan konsentrasi 2,4-D 1 mg/l dan Media ½ MS (D2M2)
dapat meningkatkan penilaian kalus, bobot segar kalus, dan bobot kering kalus. Konsentrasi
2,4-D 1 mg/l memiliki hari muncul kalus tercepat dan persentase hidup terbesar. Media ½
MS memiliki hari muncul kalus tercepat dan persentase hidup terbesar.
Kata kunci : Kalus wortel, 2,4-D, media MS.v
INDUCTION GROWTH OF CARROT CALLUS (Daucus carota L.) WITH
VARIOUS CONCENTRATIONS OF 2,4-D IN MS MEDIA COMPOSITION IN
VITRO
By : Hanada Yogia Tama (134170145)
Supervised by : Endah Wahyurini, S.P., M.Si. and Ir. Lagiman, M.Si.
ABSTRACT
Carrot (Daucus carota L.) is an important plant with high economic value. In conventional carrot
propagation, there are obstacles, example tubers that rot easily during the rainy season, tubers are
susceptible to disease, late blight, weed attacks that inhibit carrot growth, and non-uniform yield
of carrot tubers. Conventional carrot propagation problems can be overcome by using in vitro
propagation. The purpose of this study were to determine the interaction between 2,4-D treatment
and MS medium composition in inducing carrot callus growth in vitro, determine the appropriate
concentration of 2,4-D to induce carrot callus growth in vitro, and determine MS media
composition appropriate in inducing carrot callus growth in vitro. This research was conducted at
the Biotechnology Laboratory of UPN "Veteran" Yogyakarta. The research method use was
Completely Randomized Design (CRD) with two factors. The first factor is 2,4-D concentration
consist of 3 levels (0,5 mg/1, 1 mg/, and 1,5 mg/1). The second factor is compositions MS media
consist of 3 levels (¼ MS, ½ MS, and MS). Data were analyzed in analysis of variance at 5%
significance level and cuntinued by Duncan’s Multiple Range Test (DMRT). The combination of
2,4-D 1 mg/l concentration and ½ MS media can increase callus scoring value, callus fresh weight,
and callus dry weight. The concentration 2,4-D of 1 mg/l had the fastest callus appeared and had
the highest percentage of life. ½ MS media had the fastest callus appeared and had the highest
percentage of life.
Key words : Carrot callus, 2,4-D, MS media.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Kalus wortel, 2,4-D, media MS
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Eko Yuli
Date Deposited:	02 Feb 2022 06:43
Last Modified:	15 Aug 2022 07:51
URI:	http://eprints.upnyk.ac.id/id/eprint/28141

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