Karjadi, Asih K. (2020) The Effect Of Phytohormone Picloram And Bap On Shallot Meristematic Proliferation. In: Proceedings The 4th International Conference on Green Agro-Industry, 22-23 Oktober 2019, Grand Inna Malioboro.
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Abstract
Shallot (Allium ascolonicum L) is one of Allium species that is vegetatively propagated. In vitro/micropropagation has been carried out for virus free-seed production. The research was conducted at the Vegetable Research Institute Tissue Culture Laboratory started from August until December 2018. The research goal was to observe the effect of phytohormon picloram and BAP on the growth of meristematic cv. Maja. Totally 9 (nine) treatments were resulted from modified composition e.g. basic MS media (1962) + MS vitamins + sucrose 30 g / l + IAA 2 mg / l + kinetin 2 mg / l + GA3 0.01 mg / l + Myo inositol 100 mg / l + CaP 2 mg / l + gel rite 2 g / l, pH 5.7, combined with phytohormon picloram (0,1,2 mg / l) and BAP (0,1,2 mg / l). Parameters observation showed that (1) percentage of contamination until 8 WAP was 30- 50 %, generally caused by fungi or bacteria, (2) percentage of visual proliferation was between 65 - 100%, (3) explant growth in M1 to M9 media was one shoot per explant. DAS ELISA detected that tested plants were infected with OYDV, SYSV 36.36% - 53.85%. Keywords: Shallot (Allium ascolonicum L), picloram, BAP, MS Media.
| Item Type: | Conference or Workshop Item (Paper) |
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| Uncontrolled Keywords: | Shallot (Allium ascolonicum L), picloram, BAP, MS Media. |
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Engineering Sciences |
| Depositing User: | S.P., M.Sc Danar Wicaksono |
| Date Deposited: | 25 Jun 2020 07:29 |
| Last Modified: | 25 Jun 2020 07:29 |
| URI: | http://eprints.upnyk.ac.id/id/eprint/22865 |
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