Mahreni, Mahreni and KHOLISOH, Siti Diyar and Pawignyo, Harso and Yanisworo, Wijaya Ratih (2014) Production of Biohydrogen and bioacetic acid by using isolates of Bacillus Cirulans. In: The Regional Conference on Chemical Engineering 2014, Yogyakarta Decenber 02-03, 2014, Hotel Melia Yogyakarta.
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Abstract
Abstract Biohydrogen and bioacetic acid are very important chemicals. Both of these chemicals can be produced through anaerobic fermentation by using Bacillus circulans. Bacillus sp can be isolated from biogas solid sludge by growing it in the modified media. The isolation was carried out by boiling the biogas sludge for 2 hours. After it boiled, only the spores-producing microbes would survive, such as Bacillus sp and Clostridium sp. Therefore, spores were grown in hydrogen media with glucose as a carbon source, for 72 hours, in anaerobic conditions and at 60 °C. During the growth, hydrogen produced was analyzed by using gas chromatography (GC), whereas metabolite liquid products were analyzed by using HPLC to determine the composition of organic acids and other products quantitatively. Finally, the identification of microbes producing hydrogen was conducted through the PCR test. The results of the analysis proved that the microbes matched to Bacillus circulans with 97% similarity. The results of GC showed that the hydrogen was produced by Bacillus circulans. The highest content of hydrogen was found in samples 8 and 9 (SC IV). They contained acetic acid of 7.800 ppm, propionate acid of 53 ppm. PCR test by comparing the 16S rDNA gene sequences through Blast program showed that the three isolates were most likely identified as Bacillus circulans (first isolate) and Bacillus sp (second and third isolates), with 97% similarity. Bacillus circulans was known as a microbe producing enzyme. Therefore, it could be concluded that this study has successfully found a species of Bacillus circulans with a new strain that was able to produce hydrogen and has not been previously discovered. Key Words: Anaerobic; Bacillus circulans; bioacetic acid; biohydrogen; PCR; thermophilic
| Item Type: | Conference or Workshop Item (Paper) |
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| Uncontrolled Keywords: | Anaerobic; Bacillus circulans; bioacetic acid; biohydrogen; PCR; thermophilic |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Chemistry |
| Depositing User: | Dr Mahreni Mahreni |
| Date Deposited: | 23 Apr 2019 02:40 |
| Last Modified: | 23 Apr 2019 02:40 |
| URI: | http://eprints.upnyk.ac.id/id/eprint/19427 |
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