IN VITRO REGENERATION OF CHRYSANTHEMUM CALLUS AFTER GAMMA RAY IRRADIATION FOR ITS RESISTANCE TO MEDIUM PLAINS

wijayani, Ari and Muafi, Muafi (2016) IN VITRO REGENERATION OF CHRYSANTHEMUM CALLUS AFTER GAMMA RAY IRRADIATION FOR ITS RESISTANCE TO MEDIUM PLAINS. Information an International Interdisciplinary Journal, 19 (6A). pp. 1813-1818. ISSN 1343-4500 (print) ISSN 1344-8994 (electronic)

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Abstract

Development of chrysanthemum plants in medium plains is still facing obstacles due to unfavorable climate. Up until today, the availability of tolerant chrysanthemum seeds grown in medium plains is not maximized as a necessary effort in order to increase the genetic diversity available for genetic materials as the material selection to obtain tolerant chrysanthemums grown in medium plains. Researchers have been conducting studies at several stages in order to follow up the problems regarding the availability of tolerant chrysanthemums seeds grown in medium plains, one of which is the in vitro regeneration of chrysanthemum callus after gamma ray irradiation. Studies have been conducted in the tissue culture laboratory of the Faculty of Agriculture, Universitas Pembangunan Nasional"Veteran" Yogyakarta from February to June 2015. The regeneration media tested is ½ MS with the addition of IAA 0.1 mg/l; 0.2 mg/l; 0.3 mg/l; 0.4 mg/l and 0.5 mg/l. The results showed that ½ of MS regeneration media with the addition of IAA 0.3 mg/l stimulated shoots growth with a percentage of 100%; with a growth time of 9,33 days, shoots height of 11,17 cm, and a total number of shoots of 15,11. The total number of roots grown given by the regeneration medium treatment with kinetin 2 mg/l + IAA 0.4 mg/l is 12,1.

Item Type: Article
Subjects: S Agriculture > SB Plant culture
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: ARI WIJAYANI -
Date Deposited: 30 Oct 2017 01:38
Last Modified: 30 Oct 2017 01:38
URI: http://eprints.upnyk.ac.id/id/eprint/13532

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