Mahreni, Mahreni and Siti Dyar Kholisoh, Dyar and Harso Pawignyo, Pawignyi and Yanisworo Wijaya Ratih, Wijaya Ratih (2014) Producton of Biohydrogen an Bioacetic by using Isolates of Bacillus circulans. In: Regional Converence of Chemical Engineering, 2-3 Desember, Yogyakarta.
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Abstract
Producton of Biohydrogen an Bioacetic by using Isolates of Bacillus circulans Mahreni, Siti Dyar Kholisoh, Harso Pawignyo, Yanisworo Wijaya ratih Abstact Biohydrogen and bioadictic acid are very importand chemicals. Both of these chemicals can be produced thourght anaerobic fermentation by using Bacillus Circulans. Bacilllus sp can be isolate from biogas solid sludge by growing it in the modified media. The isolation wa carried out of boilling the biogas sludge for 2 hours. After it boiled, only the porre-producing microbes would survive, such as Bacillus sp and Clostridium sp. Therefore, spores were grown in hydrogen media with glucose as a carbon source, for 72 hours, in anaerobic conditions and at 60 °C. During the growth, hydrogen produced was analyzed by using gas chromatography (GC), whereas metabolite liquid products were analyzed by using HPLC to determine the composition of organic acids and other products quantitatively. Finally, the identification of microbes producing hydrogen was conducted through the PCR test. The result of the analisis proved that the microbes matced to Bacillus circulans with 97% similarity. The result of GC showed that the hydrogeen was produced by Bacillus circulans. The highest content of Hydrogen was found in samples 8 and 9 (SC IV). They contained acetic acid of 7.800 ppm, propionate acid of 53 ppm. PCR test by comparing the 16S rDNA gene sequences throught Blast program showed that the three isolates ware most likely identified as Bacillus circulans (first isolate) and Bacillus sp (second and third isolated), with 97% similarity. Bacillus circulans wan known as a microbe producing enzyme. Therefore, it could be concluded that this study succesfull found a species of Bacillus circullans with a new was able to produce hydrogen and has not been previously discovered. Keyword: anaerobic; bacillus circulans; bioacetic acid; biohydrogen; PCR; thermophilic
| Item Type: | Conference or Workshop Item (Paper) |
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| Uncontrolled Keywords: | anaerobic; bacillus circulans; bioacetic acid; biohydrogen; PCR; thermophilic |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Engineering Sciences |
| Depositing User: | MAHRENI MAHRENI MAHRENI |
| Date Deposited: | 12 Oct 2017 01:06 |
| Last Modified: | 12 Oct 2017 01:06 |
| URI: | http://eprints.upnyk.ac.id/id/eprint/13219 |
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