MAHRENI, Ahmad and RATIH, Yanisworo Wijaya and KHOLISOH, Siti Diyar and PAWIGNYO, Harso (2015) Isolation of Hydrogen Producing Bacteria from Sludge of Anaerobic Biogas Reactor. In: Proceedings The Second International Converence on Green Industry (ICGAI), Resources Management for Sustainable Future, 4-6 Agustus 2015, Yogyakarta.
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Abstract
Isolation of Hydrogen Producing Bacteria from Sludge of Anaerobic Biogas Reactor Mahreni, Yanisworo Wijaya Ratih, Siti Diyar Kholisoh, Harso Pawignyo Abstract Hydrogen-producing bacteria can be found in the activated sludge generation from anaerobic waste treatmen process. Mokrobes in it consists of acetogenesis, methanogenesis and hydrogen-producing bacterisa such as Bacillus and clostridium. The purpose of the study was to isolate, characterize and identify a hidrogen-producing bacteria, using a gas chromatograph (GC-Shimadzu). Organics acid and ethanol productions were analyzed by HPLC. Whereas reducing sugar was analysis by DNs method. Isolateds identification was done based in the 16S rDNA gene sequences. Fave isolates using liquid minerals medium (HM medium). Based on examination on the similarities of the isolates morphology obtained, three isolated have been selected. The were BYM1, BYM2 and BYM3. According to the squence of their 16S-rDNA, the BYM1, BYM2 and BYM3 were identified as Bacillus circulans with 97% similarities. The three isolate bacteria were then designted as Bacillus circullans BYM1, Bacillus circulans BYM2 and Bacillus circullan BYM3. The acid production by the isolate and their combination was higher than th C4 culture. The mixed cultures hydrigen than the C4 culture. The fermentation by-products were ethanol, ecetic acid and propionic acid, and acetic acid is the major metabolite. Keywords : biogas sludge, Bacillus, bio-hidrogen.
| Item Type: | Conference or Workshop Item (Paper) |
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| Uncontrolled Keywords: | Biogas sludge, bacillus, byohidrogen |
| Subjects: | T Technology > TS Manufactures |
| Depositing User: | MAHRENI MAHRENI MAHRENI |
| Date Deposited: | 02 Oct 2017 04:27 |
| Last Modified: | 02 Oct 2017 04:27 |
| URI: | http://eprints.upnyk.ac.id/id/eprint/12771 |
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